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Structured Review

Proteintech mtor
Mtor, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 1021 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/anti+mtor/mTOR+Antibody/pm42908476-62-34-36
Average 96 stars, based on 1021 article reviews
mtor - by Bioz Stars, 2026-11
96/100 stars

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Related Articles

Incubation:

Article Title: A multimodal ROS logic-gated therapeutic platform disrupts the vicious cycle of senescence to promote aged bone defect repair
Article Snippet: .. Membranes were blocked with 5% BSA in TBST for 1 h at 25 °C, followed by overnight incubation at 4 °C with the following primary antibodies: anti-GAPDH (Proteintech, 60004-1-Ig; 1:50,000), anti-LC3 (Proteintech, 14600-1-AP; 1:3000), anti-mTOR (Proteintech, 66888-1-Ig; 1:25,000), and anti-phospho-mTOR (Proteintech, 67778-1-Ig; 1:6000). .. Membranes were blocked with 5% BSA in TBST for 1 h at 25 °C, followed by overnight incubation at 4 °C with the following primary antibodies: anti-GAPDH (Proteintech, 60004-1-Ig; 1:50,000), anti-LC3 (Proteintech, 14600-1-AP; 1:3000), anti-mTOR (Proteintech, 66888-1-Ig; 1:25,000), and anti-phospho-mTOR (Proteintech, 67778-1-Ig; 1:6000).

Article Title: ACAT1/SOAT1 governs mitochondrial dynamics and thermogenesis via the mTOR-MFN2-USP2 axis in brown adipocytes
Article Snippet: Proteins were separated by SDS-PAGE and transferred electrophoretically to a PVDF membrane (Sigma-Aldrich, 24937-79-9). .. Membranes were incubated with primary antibodies overnight at 4 °C: anti-ACAT1 (Purified by our lab), anti-HSP90 (ProteinTech, 60318-1-Ig), anti-Beta Actin (ProteinTech, 66009-1-Ig), antiOXPHOS (Abcam, ab110413), anti-UCP1 (Abcam, ab10983), antiCalnexin (ProteinTech, 10427-2-AP), anti-IP3R (ProteinTech, 19962-1- AP), anti-FACL4 (Abcam, ab155282), anti-VDAC (ProteinTech, 10866-1- AP), anti-Tubulin (ProteinTech, 66031-1-Ig), anti-MFN2 (ProteinTech, 12186-1-AP), anti-MFN1 (ProteinTech, 66776-1-Ig), anti-OPA1 (ProteinTech, 27733-1-AP), anti-DRP1 (ProteinTech, 12957-1-AP), antipSer616-DRP1 (Cell Signaling, 3455), anti-MFF (ProteinTech, 17090-1- AP), anti-Flag (Sigma, F1804), anti-P4D1 (Santa Cruz, sc-8017), anti-HA (ProteinTech, 51064-1-AP), anti-mTOR (ProteinTech, 66888-1-Ig), antipSer2448-mTOR (Cell signaling, 5536), anti-pThr389-p70S6Kinase (Cell signaling, 9234), anti-USP2 (ProteinTech, 10392-1-AP) and antiGNPAT (ProteinTech, 14931-1-AP). .. After incubation with primary antibodies, membranes were washed with TBST and incubated with secondary antibodies conjugated to horseradish peroxidase (HRP; Epizyme, LF101 and LF102) at room temperature for 1 hour.

Cell-Signaling:

Article Title: Inducible cAMP Early Repressor Limits Glycolytic Programming in Regulatory T Cells and Supports Control of Inflammatory Colitis.
Article Snippet: Proteins were then transferred to a PVDF membrane (Bio-Rad) and incubated with primary antibodies overnight. .. For detection anti-phospho (Ser2448) mTOR (Cell Signaling, 5536) diluted 1:200 and anti-mTOR (Proteintech, 66888-1-Ig) diluted 1:200, anti-rabbit IgG (Cell Signaling, 7074) diluted 1:10,000 and anti-mouse IgG (Jackson ImmunoResearch Lab Inc., 115-036- 062) diluted 1:10,000 were used. .. As a loading control for protein samples, anti-GAPDH antibody (Cell Signaling, 5174) diluted 1:5000 was applied.

Article Title: ACAT1/SOAT1 governs mitochondrial dynamics and thermogenesis via the mTOR-MFN2-USP2 axis in brown adipocytes
Article Snippet: Proteins were separated by SDS-PAGE and transferred electrophoretically to a PVDF membrane (Sigma-Aldrich, 24937-79-9). .. Membranes were incubated with primary antibodies overnight at 4 °C: anti-ACAT1 (Purified by our lab), anti-HSP90 (ProteinTech, 60318-1-Ig), anti-Beta Actin (ProteinTech, 66009-1-Ig), antiOXPHOS (Abcam, ab110413), anti-UCP1 (Abcam, ab10983), antiCalnexin (ProteinTech, 10427-2-AP), anti-IP3R (ProteinTech, 19962-1- AP), anti-FACL4 (Abcam, ab155282), anti-VDAC (ProteinTech, 10866-1- AP), anti-Tubulin (ProteinTech, 66031-1-Ig), anti-MFN2 (ProteinTech, 12186-1-AP), anti-MFN1 (ProteinTech, 66776-1-Ig), anti-OPA1 (ProteinTech, 27733-1-AP), anti-DRP1 (ProteinTech, 12957-1-AP), antipSer616-DRP1 (Cell Signaling, 3455), anti-MFF (ProteinTech, 17090-1- AP), anti-Flag (Sigma, F1804), anti-P4D1 (Santa Cruz, sc-8017), anti-HA (ProteinTech, 51064-1-AP), anti-mTOR (ProteinTech, 66888-1-Ig), antipSer2448-mTOR (Cell signaling, 5536), anti-pThr389-p70S6Kinase (Cell signaling, 9234), anti-USP2 (ProteinTech, 10392-1-AP) and antiGNPAT (ProteinTech, 14931-1-AP). .. After incubation with primary antibodies, membranes were washed with TBST and incubated with secondary antibodies conjugated to horseradish peroxidase (HRP; Epizyme, LF101 and LF102) at room temperature for 1 hour.

Purification:

Article Title: ACAT1/SOAT1 governs mitochondrial dynamics and thermogenesis via the mTOR-MFN2-USP2 axis in brown adipocytes
Article Snippet: Proteins were separated by SDS-PAGE and transferred electrophoretically to a PVDF membrane (Sigma-Aldrich, 24937-79-9). .. Membranes were incubated with primary antibodies overnight at 4 °C: anti-ACAT1 (Purified by our lab), anti-HSP90 (ProteinTech, 60318-1-Ig), anti-Beta Actin (ProteinTech, 66009-1-Ig), antiOXPHOS (Abcam, ab110413), anti-UCP1 (Abcam, ab10983), antiCalnexin (ProteinTech, 10427-2-AP), anti-IP3R (ProteinTech, 19962-1- AP), anti-FACL4 (Abcam, ab155282), anti-VDAC (ProteinTech, 10866-1- AP), anti-Tubulin (ProteinTech, 66031-1-Ig), anti-MFN2 (ProteinTech, 12186-1-AP), anti-MFN1 (ProteinTech, 66776-1-Ig), anti-OPA1 (ProteinTech, 27733-1-AP), anti-DRP1 (ProteinTech, 12957-1-AP), antipSer616-DRP1 (Cell Signaling, 3455), anti-MFF (ProteinTech, 17090-1- AP), anti-Flag (Sigma, F1804), anti-P4D1 (Santa Cruz, sc-8017), anti-HA (ProteinTech, 51064-1-AP), anti-mTOR (ProteinTech, 66888-1-Ig), antipSer2448-mTOR (Cell signaling, 5536), anti-pThr389-p70S6Kinase (Cell signaling, 9234), anti-USP2 (ProteinTech, 10392-1-AP) and antiGNPAT (ProteinTech, 14931-1-AP). .. After incubation with primary antibodies, membranes were washed with TBST and incubated with secondary antibodies conjugated to horseradish peroxidase (HRP; Epizyme, LF101 and LF102) at room temperature for 1 hour.



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